B-cell leukemia/lymphoma panel

• Alternative Names

  B lymphocyte cell surface markers

• How the test is performed

  A sample of white blood cells is obtained by drawing blood from a vein. Less commonly, the sample is taken from a bone marrow biopsy. The test also may be done on a lymph node biopsy when lymphoma is suspected.

  Blood is typically drawn from a vein, usually from the inside of the elbow or the back of the hand. The site is cleaned with germ-killing medicine (antiseptic). The health care provider wraps an elastic band around the upper arm to apply pressure to the area and make the vein swell with blood.

  Next, the health care provider gently inserts a needle into the vein. The blood collects into an airtight vial or tube attached to the needle. The elastic band is removed from your arm. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.

  In infants or young children, a sharp tool called a lancet may be used to puncture the skin and make it bleed. The blood collects into a small glass tube called a pipette, or onto a slide or test strip. A bandage may be placed over the area if there is any bleeding.

  In the laboratory, the sample of white blood cells will be tested to determine the cell type and characteristics (immunophenotyping).

• How to prepare for the test

  No special preparation is usually necessary.

• How the test will feel

  When the needle is inserted to draw blood, you may feel moderate pain, or only a prick or stinging sensation. Afterward, there may be some throbbing.

• Why the test is performed

  This test may be performed:

  • When other tests (such as a blood smear) indicate abnormal white blood cells
  • When leukemia or lymphoma is suspected
  • To determine the type of leukemia or lymphoma
• What abnormal results mean
  • B-cell lymphocytic leukemia
  • Lymphoma
• What the risks are

  Veins and arteries vary in size from one patient to another and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.

  Other risks associated with having blood drawn are slight but may include:

  • Excessive bleeding
  • Fainting or feeling light-headed
  • Hematoma (blood accumulating under the skin)
  • Infection (a slight risk any time the skin is broken)
• Special considerations

  This test is highly specialized and may not be available at all general laboratories.

• References

  Appelbaum FR. The acute leukemias. In: Goldman L, Ausiello D, eds. Cecil Medicine. 23rd ed. Philadelphia, Pa: Saunders Elsevier;2007:chap 194.

  Bierman PJ, Harris N, Armitage JO. Non-Hodgkin's lymphomas. In: Goldman L, Ausiello D, eds. Cecil Medicine. 23rd ed. Philadelphia, Pa: Saunders Elsevier;2007:chap 196.

  Kantarjian H, O'Brien S. The chronic leukemias. In: Goldman L, Ausiello D, eds. Cecil Medicine. 23rd ed. Philadelphia, Pa: Saunders Elsevier;2007:chap 195.

Review Date: 3/2/2009

Reviewed By: David C. Dugdale, III, MD, Professor of Medicine, Division of General Medicine, Department of Medicine, University of Washington School of Medicine; and Yi-Bin Chen, MD, Leukemia/Bone Marrow Transplant Program, Massachusetts General Hospital. Also reviewed by David Zieve, MD, MHA, Medical Director, A.D.A.M., Inc.